Flow cytometer 31 pages summary of contents for bd facscanto. About this manual this manual describes how to use bd facsdiva software. Bectondickinson facscalibur flow cytometer instructions. Instruments available for use at the flow cytometry core facility, institute of cell and molecular science, barts and the london, queen mary, university of london. With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with bd horizon brilliant dyes, so you can focus on what matters mostyour research. The bd facscelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. Becton dickinson facscan the bd facscanto system breaks new frontiers in flow cytometry by providing an efficient, easytouse yet powerful sixcolor benchtop analyzer. It uses fixedalignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays. Bd lsr ii users guide indiana university bloomington.
Unless otherwise noted, bd, the bd logo and all other trademarks are property of becton, dickinson and company. Modsim following is a concise users manual for the operation of modsim32. Becton dickinson facscan bench top automated flow cytometer. Autocrit centrifuge and adams microhematocrit ii centrifuge. Database contains 3 bd facscanto ii manuals available for free online viewing or downloading in pdf. All rights reserved page 5 of 33 document 246410 rev to start a new exam, enter patient information in the create new exam window. Page 1 bd high throughput sampler user s guide for the bd lsr ii bd facscanto bd facscanto. Soluble products of helminths differentially suppress dendritic cell cytokine production. Data files and workspaces stored in my documents will not be accessible to other limited users but will be available to administrator accounts. Mentor graphics reserves the right to make changes in specifications and other information contained in this publication without prior notice, and the. Find the user manual and the help you need for the products you own at manualsonline. Lotus esprit world cannot be held responsible for anything you do with this software, cable and manual. Conventions the following conventions are used in these instructions.
This manual describes how to use bd facsdiva software. The bd facsdiva software reference manual assumes that you have a working knowledge of basic microsoft windows operation. Find flow cytometer equipment for sale, or wanted from the worlds largest medical equipment marketplace. You assume total responsibility and risk associated with using this manual. Bd facsaria ii, bd facsaria iiu, bd facsaria iii, bd facsaria iiiu, special order research product sorp bd. To change your password as a limited user, go to user accounts in the control panel, and follow the instructions to change passwords. Facscan and facscalibur cleaning and shutdown instructions. It incorporates many technology advances first developed in the bd lsr ii expandable analyzer and the bd facsaria cell sorter. Find flow cytometer listings for sale or wanted on. User bulletin directions for the use of the sheathethanol filter 122018 overview these directions are for the use of the sheathethanol filter, part number 661744, for the following instruments. Rising costs, decreasing reimbursement, labor intensive manual procedures, fewer resources, and higher demands for efficiencythey are all challenges todays clinical laboratory faces. Hts customer care kits 52 pages laboratory equipment bd accuri c6 instrument manual. This manual has be produced only as a guide to using freescan and helping in diagnosing. A bd facscan flow cytometer can be used for routine research applications, immunophenotyping, and dna cellcycle analysis.
Does not support bd 4color upgrade and sorting options on facscalibur or facsort cytometers installing the flowjo collectors edition fjce. Analyzes individual cells as they pass in a moving stream through a laser beam. Flow cytometer calibration and size reference beads. The latest version of fjce can be found on the cytek development website in the products flowjo collectors edition section. Page 12 sample oring replacement the following section describes the monthly cleaning procedure. Streaminair instruments include the bd facstar plus and bd facsvantage bd biosciences immunocytometry systems, san jose, ca flow cytometers. Bd facscanto ii measuring instruments user manual manual pdf. Notice in bd cellquest pro software, choose cytometer status.
The first bd facscanto system was introduced in 2004. Although these symbols appear in color on the instrument, they are in black and white throughout this user s. Other procedures can be found in the bd facsort users. Technical center equipment manuals, parts lists, and diagrams equipment and supplies. It analyzes cells as they pass through a focused laser beam one at a time in a moving fluid stream. Bd facscanto ii manuals and user guides, laboratory. Bd facscalibur, bd facscan, bd facsort, or bd lsr flow cytometer. Because many cytometer functions are controlled by bd facsdiva software, this guide also contains information about software features required for basic cytometer setup and operation. Laboratory equipment bd facsort user manual 14 pages laboratory equipment bd facsjazz user manual. Bd facscanto ii measuring instruments user manual manual. Facscan cleaning and maintenance purdue university.
Chapter 1 using this manual this manual contains tool usage instructions. Directions for the use of the sheathethanol filter bd. Intended use becton dickinson simultest imklymphocyte is a twocolor direct immunofluorescence reagent kit for enumerating percentages of the following mature human leucocyte subsets in erythrocytelysed whole blood lwb. Bd facscanto flow cytometer reference manualk pdf4pro.
Laboratory equipment bd facscan instructions 2 pages laboratory equipment bd lsr ii user manual. The bd facsdiva software reference manual includes instructions or descriptions for installation and setup, workspace components, acquisition. We also provide consulting services on multicolor panel design and flow cytometry data analysis. This recommendation was exactly the one i found in the german version of facscan user s manual by bd. These instructions need to be carried out after the completion of your experiment. These instructions describe how to operate your bd facscalibur flow cytometer using bd facstation software. The bd cba is not recommended for use on streaminair instruments where signal intensities may be reduced, adversely effecting assay sensitivity.
Labexchange is offering you the transfer of instructions in pdf format of numerous laboratory and analysis devices free of charge. Providing excellence in accuracy, reliability and reproducibility. Learn about bd life sciences diagnostic systems qcpi manuals. Flow cytometry histograms from a bd facscan bd biosciences, san jose, ca showing fluorescence of live and dead grampositive and gramnegative bacteria stained with the baclight bacterial stains. We are bostonlocated flow cytometry core facility equipped with analyzers 5laser bd lsr ii sorp, a facscanto and a facscalibur and cell sorters 4 laser16 color facs aria ii sorp and a 5 laser25 color moflo astrios. After passing calibration, the samples were processed and 10,000 events collected. Subsequently a tube filled with destilled water was run over 1015 minutes same settings as sodium hypochlorite solution. Bd facscalibur instructions for use bd biosciences. Bd facscan anaylser at flow cytometry core facility. Sony sy3200 haps 1 and 2 bd facscan system bd cellquest guide core facscan guide. Oct 25, 2016 bd lsr ii flow cytometer quick user guide v. View online user manual manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer.
We have facsdiva, cellquest pro and flowjo analysis stations. The bd facscalibur instructions for use assumes you have a working knowledge of the mac os x operating system. This document is designed to help the newcomer download, install and navigate from freescan, which is designed to work with the uniden dma scanners. Remove black metal plate on top of sheath fluid tank push forward, then lift off. Todays bd facscanto system features a fixedalignment flow cell in the fluidics system that minimizes startup time and improves reproducibility. Reading this material and answering the questions at the end of each. Technical center equipment manuals, parts lists, and diagrams. This users guide contains the instructions necessary to operate and maintain. These fluorescently stained polystyrene microspheres are highly uniform with respect to size and fluorescence intensity figure 3, and are designed to approximately replicate the size, emission wavelength, and intensity of biological samples.
A bd facscan system the facscan system is an automated. Facscalibur system users guide v preface facscalibur is the becton dickinson immunocytometry systems bdis modular benchtop flow cytometer designed for applications ranging from routine clinical to advanced research. Although these symbols appear in color on the instrument, they are in black and white throughout this users. Contact your sales repres entative for availability of accessories and optional equipment. For information on how to operate and maintain your flow cytometer, refer to the instrument users guide. Flow cytometer 204 pages laboratory equipment bd 644787 user manual. The three main components of a flow cytometer are the fluidics, optics, and electronics figure 1 the fluidics system of a flow cytometer is responsible for transporting sample from the sample tube to the flow cell. We continue to innovate the most sensitive detection technology for researchers, utilizing side scatter technology for nanoparticle detection to. This procedure is mandatory for maintaining proper instrument operation. The bd facscanto flow cytometer reference manual assumes you have a working knowledge of basic microsoft windows operation. Technical manuals and documentation bd lsr ii octagon and trigon filter configurations most recent, 52005 bd lsr ii overview from bd biosciences, 92002 bd lsr ii user guide from bd. Soluble helminth products suppress clinical signs in.
Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 8. Bd compbeads compensation particle sets are designed to optimize compensation settings in multicolor flow cytometry. This document is for information and instruction purposes. Bd, the bd logo, bd calibrite, bd facs, bd facscalibur, bd facscanto, bd. The bd facscan flow cytometer becton dickinson was calibrated with calibrate 3 color beads becton dickinson, using facscomp 4. Read the section of the workbook or users guide specific to the operation you are. It analyzes cells as they pass through a focused laser beam one at a time in a moving.
Bd becton, dickinson and company diagnostic systems. This software is the property of becton, dickinson and. Baclight bacterial stains b35000 baclight green bacterial stain b35001 baclight red bacterial stain figure 1. Other procedures can be found in the bd facsort user s. Competition started between the different flow cytometer manufacturers concerning the simultaneous analysis capacity of fluorescence. In addition, you need the security module provided with cellquest. Bd facs clean solution is a bleachbased cleaning agent for daily use in cytometer maintenance. A bd facscan flow cytometer can be used for routine research applications, immunophenotyping, and. Bd, bd logo and all other trademarks are property of becton, dickinson and company. User manuals, guides and specifications for your bd facscanto ii laboratory equipment, measuring instruments. Invitrogen alignflow flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers. Bd lsr ii users guide describes procedures necessary to operate and maintain your bd lsr ii flow cytometer. Baclight bacterial stains thermo fisher scientific. This modular system features advanced capabilities, such as the sorting and fl4 options in an easytouse system.
Some of the illustrations shown in this manual may contain accessories and optional equipment that are not included on your system. Since then, there have been many innovations in the product line, reflecting our commitment to reach to ever higher standards. Operation of the 16bit version of modsim is similar, but is not detailed in this document. Laboratory equipment bd facscan instructions 2 pages. We offer specialized instruments for routine or high complexity flow cytometric analysis designed with a deep understanding of the clinical workflow and the needs of the hematopathologist. Bd facscanto ii manuals and user guides, laboratory equipment. Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with the sale of every new cytometer. A to perform the steps in this tutorial, you will need. For information on how to operate and maintain your flow cytometer, see your cytometer manual. How a flow cytometer works thermo fisher scientific us. At bd, we seek to usher in a new era of healthcare by bringing medical products, capabilities and solutions to every corner of the world. Once through the flow cell and past the laser, the sample is either sorted in the case of cell sorters or transported to waste.
Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. Bs lsrfortessa cell analyzer users guide bd biosciences. Microbial enumeration in 4 minutes pass fail results for presence absence of microbial contamination in 24 to 48 hours yeast, bacteria and mold detection in a single run. Flow cytometers, reagents, software beckman coulter. Flow cytometer 31 pages laboratory equipment bd csampler installation manual. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. Flow cytometers, cell sorters and cell preparation systems. National laser replacement lasers for life science. Page 1 bd high throughput sampler users guide for the bd lsr ii bd facscanto bd facscanto. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Refer to the bd facscan system users guide for troubleshooting the facscan instrument. In 1985, cell analyzers allowed up to three color analysis, such as epics c analyzer leif et al. National laser offers the widest variety of replacement lasers for life science inspection equipment.
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